This week Reem and I continued conditioning the second group of mice (Coc vs. Ms) and finished testing on the first group of mice (Ms vs. Oxy) by running the state dependent tests. On Monday before we started conditioning on the second group, Sarah taught Reem and I how to analyze the pre-conditioning data to determine which compartment each mice would get the drugs in. We use the program Ethovision to analyze the video and determine whether each mice had a bias towards a certain side in the CPP box. Before running the videos we have to adjust the box settings and make sure the mice are tracked properly bu ensuring the 'subject not found' number is below 1%.
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| Running the pre-conditioning videos on Ethovision |
After balancing out which mice would get the drug in which compartment and on which day, we turned the data into a table so that it will be easier for us to prepare the boxes and injections during the conditioning days.
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| Condensation of the pre-conditioning data |
The table tells us which drug the mice is going to get and on which side of the box. We alternate day 1 and day 2 of conditioning for 8 days in a row and then a post-conditioning test is done before the two state dependent tests. While we condition the second group of mice we started to analyze the data from the first group of mice. After repeating the same settlings for the boxes, the date is converted into an excel sheet and analyzed using Prism. In Prism we can enter the data and acquire different tables that show whether or not the data is significant for the study. Since this is the second trial of the study we still need to combine and compare it to the first trial done. We also had a lab meeting, the first one since my PI got back from vacation. We used the time to get everything on track and determine what needs to be done in the coming weeks.
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| The setup of the CPP boxes |
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