Aisha Kumar Entry #4 Creating my Poster

This week i mainly just helped out around the lab, and did work to help people work more efficiently. I helped people make and label slides to put brains on after they cut it, and prepare test tubes for other experiments. One of the post- docs, Amy, helped me edit and construct my poster for EXP. She helped me understand specifically which antibodies i used last week, and exactly what they did with the tissue. We made this chart to help tie everything together.

Primary Antibody	Secondary Antibody	Cell Type
Fluorescein-conjugated Lycoperiscon esculentum (tomato) Lectin	None	Fluorescent endothelial cells marker
Anti-Glial Fibrillary Acidic Protein GFAP (mouse)	Donkey anti-mouse 488	Fluorescent astrocytes marker
Anti-NeuN (mouse)	Donkey anti-mouse 488	Fluorescent neuron (soma) marker
Anti-CD13 (goat)	Donkey anti-goat 647	Fluorescent pericytes marker
Anti-alpha smooth muscle Actin (rabbit)	Donkey anti-rabbit 567	Fluorescet vascular  smooth muscle cells marker
Anti-Neurofilament H Non-Phosphorylated Mouse mAb (SMI-32) (mouse)	Donkey anti-mouse 488	Fluorescent Neuron (axon) marker
Anti-IBA I (rabbit)	Donkey anti-rabbit 567	Fluorescent microglia marker

I took more pictures of the slides I stained last week, and made a presentation which includes the pictures, and what it shows the hippocampus,  and the axons and neurons within the brain.

Gwyneth also came this past weekend, and it was  fun to show her around LA with Nicki, as well as have a fun fourth of July together! I cant believe there is only one week remaining!